Category Archives: Biotechnology

What is Southern Blotting? How it is used to identify Specific DNA Sequences?

Southern Blotting is an elegant technique that can be used to identify the locations of genes and other DNA sequences on restriction fragments separated by gel electrophoresis. This technique is named after the Edwin Southern (1975) who developed it. Continue reading What is Southern Blotting? How it is used to identify Specific DNA Sequences?

What are SNPs? Why are Scientists interested in them?

A most common and important source of genetic variability is known to be present uniformly throughout the genome is termed Single Nucleotide Polymorphisms or SNPs. Interest in SNPs lies in the fact that these polymorphisms may be responsible for the differences in disease susceptibility, drug metabolism and response to environmental factors between individuals. Even, if they are not directly responsible for the disease, they serve as genetic markers for a nearby locus that might be responsible. Continue reading What are SNPs? Why are Scientists interested in them?

How a 2 meters long DNA is fitted into a 2 micrometers Nucleus?

An average Human cell (diploid) contains about 6.4 billion base pairs of DNA divided among 46 chromosomes. The length of each base pair is about 0.34 nm. Therefore, if the DNA molecule in a diploid cell were laid out end to end, the total length of DNA would be approximately 2 meters. Continue reading How a 2 meters long DNA is fitted into a 2 micrometers Nucleus?