Category Archives: Biotechnology

Personal Genomics: Gene Sequencing & Analysis in India.

In recent times, the people of India have been heard talking about gene sequencing, gene editing, gene sequencing and many other similar terms. It seems like, they are now getting introduced to the idea and principle behind personal genomics. Continue reading Personal Genomics: Gene Sequencing & Analysis in India.

What is Southern Blotting? How it is used to identify Specific DNA Sequences?

Southern Blotting is an elegant technique that can be used to identify the locations of genes and other DNA sequences on restriction fragments separated by gel electrophoresis. This technique is named after the Edwin Southern (1975) who developed it. Continue reading What is Southern Blotting? How it is used to identify Specific DNA Sequences?

What are Explants?

Explants are small pieces of plant parts or tissues that are aseptically cut and used to initiate a culture in a nutrient medium. Explants can be taken from different parts of a plant such as shoots, leaves, stems, flowers, roots, and from many types of mature cells provided they are able to de-differentiate into totipotent cells. Continue reading What are Explants?

What is Plant Tissue Culture?

Plant Tissue Culture is a technique of growing cells, tissues or organs in sterilised nutrient media under controlled aseptic conditions. The plant material to be cultured may be cells, tissues or plant organs such as excised root tip, shoot tip, shoot bud, leaf petiole, inflorescence, anther, embryo, ovule or ovary. Continue reading What is Plant Tissue Culture?

What are SNPs? Why are Scientists interested in them?

A most common and important source of genetic variability is known to be present uniformly throughout the genome is termed Single Nucleotide Polymorphisms or SNPs. Interest in SNPs lies in the fact that these polymorphisms may be responsible for the differences in disease susceptibility, drug metabolism and response to environmental factors between individuals. Even, if they are not directly responsible for the disease, they serve as genetic markers for a nearby locus that might be responsible. Continue reading What are SNPs? Why are Scientists interested in them?